Few studies have been conducted on Stone pine (Pinus pinea) morphology, cone to kernel yield (KY) and composition. To study the species variability, a morphometric and chemical characterization of pine nuts was performed across the species distribution in Chile. Seventy-six highly productive trees were selected in three macrozones. Ten cones per tree were harvested, and cone weight, in-shell pine nut number cone−1 and size and weight of in-shell pine nuts and kernels were measured; KY was determined. Chemical and nutritional characterization of pine nuts was performed in 38 trees. Differences among macrozones were tested with mixed linear models. Partial correlation was used to test for correlation between cone and pine nut morphometric data, chemical features, and climate. Canonical correlation coefficients between groups were tested. Selected trees differed in cone and in-shell pine nut size, kernel morphometry, and KY, with lowest trait values being generally found in the coast. KY was higher than in Europe. Chemical composition was similar to values reported in producer countries, homogeneous among macrozones, with the exception of oleic acid and potassium. Detected differences would respond to the environment and to the interaction genotype × environment given the restricted origins of the genetic material, confirming the species adaptability.
